| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1511 |
| 발표분야 |
생물화공 |
| 제목 |
Fabrication of Uniform DNA-Conjugated Hydrogel particles via Replica Molding for Facile Nucleic Acid Hybridization Assays |
| 초록 |
We identify and investigate several critical factors in the fabrication of single-stranded DNA conjugated poly-(ethylene glycol) (PEG) microparticles based on replica molding (RM) for highly uniform and robust nucleic acid hybridization assays. The effects of PEG-diacrylate, probe DNA, and photoinitiator concentrations on the overall fluorescence and target DNA penetration depth upon hybridization are studied. Fluorescence and confocal microscopy results illustrate high conjugation capacity of the probe and target DNA, femtomole sensitivity, and sequence specificity. Combined, these findings demonstrate a significant step toward simple, robust, and scalable procedures to manufacture highly uniform and high-capacity hybridization assay particles in a well-controlled manner by exploiting many advantages that the batch processing-based RM technique offers. We expect that the results presented here may be readily applied to rapid and high-throughput hybridization assays for a wide variety of applications in bioprocess monitoring, food safety, and biological threat detection. |
| 저자 |
최창형1, Christina L. Lewis2, Yan Lin3, 이현민4, 이창수3
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| 소속 |
1Dept. of Chemical Engineering, 2Chungnam National Univ., 3Dept. of Chemical and Biological Engineering, 4Tufts Univ. |
| 키워드 |
Hydrogel particles; Nucleic acid hybridization assay; shape-based encoding
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
