| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 가을 (10/21 ~ 10/23, 일산 KINTEX) |
| 권호 |
21권 2호, p.1735 |
| 발표분야 |
생물화공 |
| 제목 |
Biofuel (gasoline) production by metabolically engineered Escherichia coli |
| 초록 |
A platform E. coli strain for the productions of short-chain alkanes, FFAs, fatty esters, and fatty alcohols was developed. The fadE gene was deleted to prevent the degradation of fatty acyl-CoAs. The activity of FebH was promoted for the enhancement of the initiation of fatty acid biosynthesis by fadR gene deletion. A mutant TesA converted short chain fatty acyl-ACPs to the corresponding FFAs. The sequential reactions of fatty acyl-CoA synthetase, fatty acyl-CoA reductase, and fatty aldehyde decarbonylase converted the short chain FFAs into corresponding alkanes. The engineered E. coli strain produced up to 580.8 mg/L of short chain alkanes. [This work was supported by the Advanced Biomass Research and Development Center of Korea (NRF-2010-0029799) through the Global Frontier Research Program of the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF). Systems metabolic engineering work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) by MSIP through NRF]. |
| 저자 |
이상엽1, 최용준2, 하지연3
|
| 소속 |
1MBEL, 2Department of Chemical and Biomolecular Engineering, 3KAIST |
| 키워드 |
Short chain alkane; Metabolic engineering; Gasoline; biofuel
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
