| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 가을 (10/21 ~ 10/23, 일산 KINTEX) |
| 권호 |
21권 2호, p.1739 |
| 발표분야 |
생물화공 |
| 제목 |
Metabolic engineering of Corynebacterium glutamicum to overproduce L-arginine |
| 초록 |
We report a metabolically engineered Corynebacterium glutamicum strain for L-arginine production. Random mutagenesis was first performed to improve tolerance to L-arginine. Inactivation of arginine operon repressors, reinforced PPP flux, deletion of the Ncgl1221 exporter gene, optimized expression levels of argF and carAB and argGH operon overexpression resulted in 81 g/L of L-arginine production in a 1,500 L bioreactor under fed-batch conditions. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA001-2012M1A2A2026556).] |
| 저자 |
이상엽1, Suok-su Kim2, Jun Seok Park3, Tae Yong Kim4, Hyun Uk Kim5, Seok Hyun Park6, Ziwei Luo7
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| 소속 |
1MBEL, 2Dept. of Chemical and Biomolecular Engineering (BK21 Plus rogram), 3KAIST / BioInformatics Research Center, 4Institute for the BioCentury, 5BioProcess Engineering Research Center, 6KAIST, 7Daesang corporation research center |
| 키워드 |
Rational metabolic engineering; Corynebacterium glutamicum; L-arginine; Random mutagenesis; Industrial-scale fed-batch fermentation
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| E-Mail |
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| 원문파일 |
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