| 학회 | 한국공업화학회 |
| 학술대회 | 2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 | 19권 1호 |
| 발표분야 | 생물공학_포스터 |
| 제목 | Selective control of gene expression in a cell-free protein synthesis system using antisense oligonucleotides |
| 초록 | In this study, we describe the use of antisense oligonucleotides for selective repression of gene expression in a cell-free protein synthesis system. When the selected antisense oligonucleotides were added to a reaction mixture of cell-free protein synthesis that had been programmed with multiple template DNAs, expression of the target genes carrying the complementary sequence were selectively repressed. Moreover, the antisense oligonucleotides were able to be used as a reversible switch to control the expression of target genes by conjugating them with a temperature-responsive polymer. Presented results suggest that expression of recombinant proteins can be modulated by physical stimuli and the possibility of modulating the expression of individual genes in the context of complicated networks. |
| 저자 | 민승의, 김동명 |
| 소속 | 충남대 |
| 키워드 | Cell-free protein synthesis; Antisense oligonucleotide |
