| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2694 |
| 발표분야 |
생물화공 |
| 제목 |
Expression and Characterization of Hybrid Mussel Adhesive Protein FP-151 in Recombinant Baculovirus/Insect Sf9 Cell System |
| 초록 |
Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives which can adhere in aqueous conditions. Recently, we construct a functional recombinant hybrid mussel adhesive protein fp-151 that is a fusion protein comprising six-repeated fp-1 decapeptide repeats at both each terminus of fp-5 terminus and express the protein in E.coli system. However there cannot be happened post translational modification in E. coli system, so there are no 3, 4-dihydroxyphenyl-L-alanine (DOPA) and phosphorylated serine, which is known that is very important on adhesion process of mussel foot protein. For expression of originally modified fp-151 similar with natural form, we produced a recombinant fp-151 in baculovirus/insect Sf9 cell expression system and checked the DOPA and phosphorylated fp-151 formation. Through this difference between fp-151 in inset Sf9 and E.coli, we could characterize functionality and originality of our novel model mussel adhesive protein. |
| 저자 |
임성혜1, 김경로1, 황동수2, 최유성1, 차형준1
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| 소속 |
1포항공과대, 2UCSB |
| 키워드 |
Mussel adhesive protein; bioadhesive; hybrid fp-151; baculovirus; insect Sf9 cells
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| E-Mail |
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| 원문파일 |
초록 보기 |
