| 학회 | 한국화학공학회 |
| 학술대회 | 2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 | 20권 1호, p.1018 |
| 발표분야 | 환경 |
| 제목 | Development of biocatalysts for biomimetic CO2 capture |
| 초록 | Carbonic anhydrase (CA) is an enzyme that catalyzes reversible hydration of carbon dioxide into bicarbonate and proton. It has been recently suggested that this remarkably fast enzyme can be used for sequestration of CO2, a major greenhouse gas, making this a promising alternative for chemical CO2 mitigation. For its practical application, we developed efficient and economic biocatalysts with high stabilization, based on large production of a recombinant CA from Neisseria gonorrhoeae (ngCA) in Escherichia coli. First, we engineered ngCA in the periplasm of E. coli to promote the economical use of enzymes, thereby creating a bacterial whole-cell catalyst. We then investigated the application of this system to CO2 sequestration by mineral carbonation, a process with the potential to store large quantities of CO2. Next, we developed and characterized bioinspired silica nanoparticle with auto-encapsulated recombinant ngCA. The silica formation was mediated by the silica-condensing R5 peptide fused to the ngCA. This bioinspired silica nanoparticle with CA can be efficiently applied to CO2 sequestration with the outstanding entrapment, catalytic performance, and stability. |
| 저자 | 조병훈, 차형준 |
| 소속 | 포항공과대 |
| 키워드 | carbonic anhydrase; carbon capture; whole cell biocatalyst; silica encapsulation |
| 원문파일 | 초록 보기 |
