| 초록 |
Carbonic anhydras (CA) is a potential biocatalyst for CO2 sequestration because of its ability to catalyze the conversion of CO2 to bicarbonate. High production and immobilization of functional CA are required for practical application of enzymatic CO2 capture system. In previous report, α type-CA (HC-aCA) of Hahella chejuensis KCTC was mostly produced as insoluble form in E. coli expression system. Here, by removal of the signal peptide (sp), we successfully set up soluble expression systems. Under the optimized conditions, 30 mg/L of purified HC-aCA(sp-) was obtained. HC-aCASP(-) displayed high pH stability in alkali condition with maximal activity at pH 10. In addition, we chemically immobilized the HC-aCA(sp-) on silica particles. HC-aCASP(-) immobilized silica particle activity retained above 90% even after 10 times reuse. |
