| 초록 |
Protein adsorption of polymeric surfaces has been widely explored to improve performance in field of purification, biosensor, and other biomedical applications. A number of nanostructured materials have been used as advanced protein adsorbents. Especially, electro-spun polymeric nanofibers with an intrinsic affinity for target protein have high potential due to their high specific surface area. In this study, we report novel polymeric nanofiber membranes for detection of a toxic peptide. As the target peptide, melittin has six positively charged amino acids and many hydrophobic groups. Therefore, negatively charged acrylic acid (AA) and hydrophobic N-tert-butylacrylamide (TBAm) were selected as the functional monomers. As a backbone monomer, N-isopropylacrylamide (NIPAm) was employed to improve hydrophilcity of polymer nanofibers. Various poly(NIPAm-co-TBAm-co-AA) nanofibers with different monomer ratio were synthesized and their adsorption properties for melittin were investigated. |
