| 초록 |
Small interfering RNA (siRNA) has been widely researched as a potential therapeutics field for treatment of various diseases, including cancer and viral infection. However, the delivery of siRNA should overcome significant obstacles for efficient delivery due its limited stability. Thus appropriate delivery carriers are required for successful siRNA delivery. We hypothesized that glycol chitosan modified with positively charged oligopeptide (R9G10) could be useful to enhance the stability glycol chitosan/siRNA complexes and to improve the transfection efficiency. Various physicochemical characterics of oligopeptide-modified glycol chitosan derivative/siRNA complexes, including particle size, surface charge, stability, capacity of complexation, cytotoxicity, and in vitro transfection efficiency, were investigated. This approach to developing a novel delivery system using natural polymers may provide a useful means to many gene delivery applications. |
