| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Structural characterization of phosphoethanolamine-modified lipid A from probiotic Escherichia coli strain Nissle 1917 |
| 초록 |
Gut microbiota, a complex microbial community inhabiting human or animal intestines recently regarded as an endocrine organ, has a significant impact on human health. Escherichia coli (E. coli) strain Nissle 1917 (EcN), a Gram-negative probiotic bacterial strain, has been used to treat gastrointestinal (GI) disorders. However, endotoxicity of lipopolysaccharide (LPS), a major component of the cell wall of Gram-negative bacteria in the gut, is known to have a strong influence on gut inflammation and maintenance of gut homeostasis. In this study, MALDI multiple-stage mass spectrometry analysis of lipid A extracted from EcN demonstrates that EcN contains phosphoethanolamine (PEA)-modified lipid A. These results indicate that EcN can more efficiently colonize the intestine through PEA-mediated tolerance despite the presence of cationic antimicrobial peptide in human gut. |
| 저자 |
조성현1, 박한규1, 송원석2, 김성민1, 전효진1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
MALDI MS; probiotics; Escherichia coli strain Nissle 1917; lipid A; phosphoethanolamine; cationic antimicrobial peptide
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| E-Mail |
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