| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Target Specific Gene Silencing of Layer-by-Layer Assembled Gold-Cysteamine / siRNA / PEI / HA Complex |
| 초록 |
We successfully developed cysteamine modified gold (AuCM) / siRNA / polyethyleneimine (PEI) / hyaluronic acid (HA) complexes using layer-by-layer method for target specific intracellular delivery of siRNA by HA receptor mediated endocytosis. AFM and zeta potential analyses confirmed the formation of AuCM/siRNA/PEI/HA complex having a particle size of ca. 38 nm with slightly negative surface charge characteristics. Microscopic images and dark-field bioimaging clearly visualized the target specific intracellular delivery of AuCM/siRNA/PEI/HA complex to B16F1 cells with HA receptors such as CD44 and LYVE-1. With negligible cytotoxicity, AuCM/siRNA/PEI/HA complex showed an excellent target specific gene silencing efficiency higher than 70% in B16F1 cells in the presence of 50 vol% serum. Moreover, systemic delivery of AuCM/siRNA/PEI/HA complex by tail vein injection to Balb/c mice resulted in effective gene silencing with a significantly reduced apolipoprotein B mRNA level in the liver. |
| 저자 |
이민영1, 박상준2, 박기태2, 김기수2, 한세광2
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| 소속 |
1포항공과대, 2포항공대 |
| 키워드 |
Gold; siRNA; Polyethyleneimine; Hyaluronic acid; Target delivery
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| E-Mail |
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