| 초록 |
An artificial construct mimicking the intrinsic properties of the natural extracellular matrix in bones has been considered an ideal platform for bone tissue engineering. In this study, we introduce biodegradable scaffolds consisting of Polycaprolactone(PCL). We frbricated PCL nanofibers using electrospinning. The ability of the scaffolds to facilitate osteogenic differentiation was investigated using human mesenchymal stem cell(hMSC). To increase the cell adhesion and proliferation, we introduced amine group was grafted on the scaffolds using gamma-irradiation and then heparin immobilized scaffolds using EDC/NHS reaction. The properties of the nanofibers were analyzed with SEM, ATR-FTIR, Toulidine blue O(TBO) assay, fluorescamine, water contact angle measurement, and X-ray photoelectron spectroscopy(XPS), general cell responses on the scaffolds, such as adhesion and proliferation were also investigated using hMSC by SEM, fluorescent images, CCK-8. During the hMSC culture, the initial viability of the cells were significantly increased on the 2-aminoethyl methacrylate(AEMA) grafted scaffolds. After loaded BMP-2, the result of release test, heparin immobilized scaffolds were stabilized to release by ELISA. Therefore, heparin immobilized scaffolds may give a chance as a alternative tool for bone tissue engineering applications. |
