| 학회 | 한국화학공학회 |
| 학술대회 | 2006년 가을 (10/27 ~ 10/28, 고려대학교) |
| 권호 | 12권 2호, p.1759 |
| 발표분야 | 생물화공 |
| 제목 | Development and proteom analysis of recombinant E.coli strain Crds-C(836bp) which produces soluble beta-1,3-glucan |
| 초록 | Polysaccharides, particularly glucans, have a long history as immuno -modulators1). β-1,3-glucans have been found to induce the activation of different defence processes in both plants and animal2). β-1,3-glucans are a heterogeneous group of glucose polymers found in the cell walls of plant, yeast, bacteria and fungi. Following β-1,3-glucan synthesis metabolic pathway, UDP-glucose is the exclusive precursor for the synthesis of β-1,3-glucan. To produce β-1,3-glucan in E. coli, recombinant E. coli strains carrying β-1,3-glucan synthase genes and producing β-1,3-glucan synthase which catalyzed the reaction from UDP-glucose to β-1,3-glucan were developed. As a result, recombinant E. coli strains[CrdS-F(1964bp), CrdS-C(836bp) and CN-termH6(1124bp), CC-termH6(1676bp)] were obtained and their glucan production characteristics were studied. E.coli mutant strain Crds-C(836bp) produces amount 5.9g/L soluble β-1,3-glucan. And then we analyzed the proteome of the E.coli mutant strain Crds-C(836bp) with 2D-electrophoresis. Acknowledgement : This research was supported by WISE center of Chosun University in GwangJu, Chonnam. |
| 저자 | 김려화, 윤춘희, 이중헌 |
| 소속 | 조선대 |
| 키워드 | e.coli mutant. soluble beta-1; 3-glucan; proteom analysis |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
