| 초록 |
Pathway optimization of microbial metabolism is essential for the production of commercially valuable chemicals such as biofuels, platform chemicals and biologically active compounds. Currently, typical approach for metabolic engineering uses both rational approach as well as non-rational methods such as combinatorial and evolutionary methods. General strategy to modify cellular physiology depending the robustness or flexibility of the biological systems should be required. In this study, we developed the general tools to modify the biological robustness at the various levels including translation and protein levels. For translation level control, a model to predict the translation efficiency based on the mRNA’s secondary structure and consequently expression level can be precisely controllable for any genes of interest. Additionally, intracellular metabolite sensor named “riboselector” to regulate metabolic distribution will be presented. The potentials of the platform technology developed in this study for the application to the production of value-added biochemical including flavonoids and isoprenoids. |
