| 초록 |
Polydeacetylene(PDA) liposomes have unique properties in environment changes. These properties allow liposomes to change and emit different fluorescence in response to environment changes such as temperature, antibody-antigen interaction, and so on. This material has been extensively researched and applied as signal transducers in biosensor applications. In this research, we demonstrated an antibody-antigen based biosensor using PDA liposomes for detection of hIgE. In case of the addition of gold nano particles-conjugated polyclonal antibody on the liposomes, fluorescent responses dramatically increased depending on target protein concentration. As the results, we could confirm that gold nano particles-conjugated polyclonal antibody affect fluorescent signal amplification of PDA liposomes chip. This strategy can be useful to detect proteins of ultra low concentration. |
