| 초록 |
DNA-templated silver nanoclusters have emerged as promising material for sensing, bio-labelling, and bio-imaging. Herein, we developed two different types of DNA-AgNCs systems for the detection of potassium ion (K+) and nitric oxide (NO) by utilizing structural change or transformation of template DNA. In K+ detection, thrombin binding aptamer was used as both template of AgNCs and binding probe of K+. The fluorescence emission of DNA-AgNCs probe was diminished proportionally to the amount of K+ detected, which transformed AgNCs-adapted structure into G-quadruplex. On the other hand, NO detection was accomplished using C12 sequence by NO-induced deamination of cytosine. Mutation of cytosine to deoxyuridine destabilized the DNA-AgNCs structure, resulting in quenching fluorescence intensity. Each system provided detection limit of 6.2 μm for K+ and 0.1 μm for NO. |
