| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1542 |
| 발표분야 |
생물화공 |
| 제목 |
Detection of food-borne pathogens using randomly selected non-sequenced genomic DNA probes-based DNA microarray |
| 초록 |
Detection of Food-borne pathogen is critical issue in public health and the food industries. Among various molecular diagnostic methods, DNA microarray technology was applied for pathogen detection. In this study, Three food-borne pathogens, Staphylococcus aureus, Salmonella enterica subsp.enterica serovar Typhimurium and Bacillus cereus, were targeted for the preparation of the DNA microarray probe, 51∼56 DNA probes selected randomly from non-sequenced genomic DNA of each pathogen were prepared by using a set of restriction enzyme pairs. This proto-type DNA microarray chip was redesigned and successfully optimized by eliminating a few cross-hybridized probes. As a Positive result was determined when the average intensity of target region was over the 3SD + threshold intensity. This microarray chip was successfully proved to be the high throughput simultaneous detection chip for the detection of food-borne pathogens, without knowing the sequence information of the target bacteria. |
| 저자 |
장관종1, 이진용2, 김병찬1, 안주명2, 구만복3
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| 소속 |
1School of Life Sciences and Biotechnology, 2Korea Univ., 3Environment Division |
| 키워드 |
Food-borne pathogen; DNA microarray chip; non-sequenced genomic DNA probes
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| E-Mail |
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| 원문파일 |
초록 보기 |
