| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Focal points-adhesive nano-pillar array for human mesenchymal stem cell (hMSC) |
| 초록 |
Cell differentiation can be controlled by manipulating focal adhesion point because cell attachment is related to cell behaviours. In order to induce differentiation of hMSCs, we prepared nano-scale pillar array modified with focal adhesion protein. The master with nanoscale-hole arrays was patterned on silicon wafer by e-beam lithography. Nano-pillar array was prepared using poly (dimethylsiloxane) (PDMS) against the master. PDMS replica was placed on a fibronectin (0.1mg/ml) coated slide and applied light pressure. After fibronectin coating, hMSCs (5x103cells/device) were cultivated for 24h. To measure the expressions of focal adhesion proteins, immunofluorescence was performed in hMSCs. The nano-pillar array was successfully fabricated at spacing of 3, 5, 10 μm with diameter of 500nm and height of 1μm on PDMS replica. Focal adhesion proteins were clearly visualized according to the spacing gap between pillars on the PDMS. Filopodias of hMSC stretched out along the top of pillars. |
| 저자 |
김혜성, 최지숙, 손영주, 윤수진, 조은주, 김영희, 유혁상
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| 소속 |
강원대 |
| 키워드 |
human mesenchymal stem cell; poly (dimethylsiloxane)
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| E-Mail |
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