| 초록 |
Klebsiella pneumoniae is able to metabolize glycerol as a sole source of carbon and energy. In the absence of and extermal oxidant, glycerol is fermented by a dismutation processs involved two pathways. Among the rest, glycerol is dehydrated by glycerol dehydratase to form 3-hydroxypropionaldehyde, which is reduced to the major fermentation product 1,3-propanediol (PDO) by the NADH-linked 1,3-propanediol dehydrogenase (PDH), thereby regenerating NAD+. PDH activity was measured spectrophotometrically (E340) at 37℃. The assay mixture contained 2mM NAD+, 30mM Ammonium sulfate, 0.1M Potassium carbonate (pH 9.7) and 0.1M PDO. PDH was purified from Klebsiella pneumoniae and determined the activity, recyclability, pH effect, and stability of immobilized enzymes. |
