| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.350 |
| 발표분야 |
생물화공 |
| 제목 |
Biosynthesis of 2-hydroxyacids containing polymers in E. coli |
| 초록 |
Polyhydroxyalkanoates (PHAs) are polyesters which have general plastic properties, biodegradability and biocompatibility. To produce various polymers for wide range of applications, many hydroxyacids are co-polymerized. One of them, 2-hydroxyacids are nonnatural substrates of the key enzyme of PHA synthesis, PHA synthase. Here, we produced lactate and 2-hydroxybutyrate (2HB) containing PHAs with evolved PHA synthase and propionyl-CoA transferase by metabolically engineered E. coli. The evolved PHA synthase from Pseudomonas sp. MBEL 6-19 could successfully synthesized lactate and 2HB containing polymers. Further engineering was done to redirect flux toward lactate and 2HB by introducing heterogenous metabolic pathway. It resulted in enhanced PLA, P(2HB), and copolymer production. [“This work was supported by the Technology Development Program to Solve Climate Changes from National Research Foundation of Korea (Development of systems metabolic engineering platform technologies for biorefineries; NRF-2012-C1AAA001-2012M1A2A2026556) and Intelligent Synthetic Biology Center (2011-0031963) of Korea through the Global Frontier Research Program of the Ministry of Education, Science and Technology (MEST).” ] |
| 저자 |
최소영1, 양정은1, 박시재2, 이승환2, 송봉근2, 이상엽1
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| 소속 |
1KAIST, 2화학(연) |
| 키워드 |
bioplastic; metabolic engineering; lactate; 2-hydroxybutyrate
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| E-Mail |
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| 원문파일 |
초록 보기 |
