| 초록 |
Poly-lactic acid (PLA) is highlighted as alternative for petroleum based plastic, and biodegradable polymer, which can be degraded by microorganism. Many researchers tried to use mixed culture for biodegradation of PLA, but only few bacteria was reported. In this study, we tried to isolate and identify the PLA degrading bacteria in mixed culture. Batch experiment was conducted anaerobically in nutrient media including PLA granule using sludge for 40 days. All flasks were replaced with fresh media every 10 days for isolation of PLA degrading bacteria. Methane and carbon dioxide production was measured for identification of PLA degradation. SEM image illustrated that microbe considered as PLA degrading bacteria was attached on PLA surface. Microbe attached on PLA granule and suspended in media was identified using 16s rRNA pyrosequencing. As a result Achromobacter pulmonis, Stenotrophomonas acidaminiphila, Ochrobactrum intermedium, Pseudomonas aeruginosa, were identified. |
