| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Quantitative analysis strategy for N-acyl homoserine lactones (AHLs) as quorum sensing modulators via MALDI-TOF MS |
| 초록 |
As N-Acyl homoserine lactones (AHLs) are used for antibacterial drug development and cell-to-cell communication, these are important secondary metabolites. Here, we proposed a highly sensitive and reliable MALDI-MS based 3-oxo AHL quantitation method, which ketone group of AHLs were derivatized with Girard’s reagent T to (GT) to produce a permanent cationic charge state [M]+. By the chemical derivatization of 3-oxo AHLs, its detection sensitivity was improved and the derivatization also allowed accurate quantitative analysis from the PAO1 culture supernatants. This method could be applied for developing high-throughput and sensitive detection methods of quorum sensing signal molecules. |
| 저자 |
황철환1, 박한규1, 송원석2, 안다희1, 정윤이1, 김성민1, 김윤곤1
|
| 소속 |
1숭실대, 2서울대 |
| 키워드 |
homoserine lactones; MALDI-MS; Girard’s reagent T; ketone
|
| E-Mail |
|
