| 학회 |
한국화학공학회 |
| 학술대회 |
2005년 가을 (10/21 ~ 10/22, 인하대학교) |
| 권호 |
11권 2호, p.2085 |
| 발표분야 |
생물화공 |
| 제목 |
Development of microfluidic device system for the bioluminescent assay of ATP |
| 초록 |
μ-TAS(micro-total analysis system) has increasing concern as an analytical device for various applications. The field of μ-TAS is rapidly developing because of its advantages. The simple fabrication techniques based on polymer and soft-lithography enhanced the usages of μ-TAS. In this study, the microfluidic device and detector system have been developed for measuring ATP based on bioluminescence. The concentration of ATP was determined by bioluminescent reaction using luciferase and home-made detector system. A serpentine-shape reactor was fabricated in PDMS microfluidic device to enhance the sensitivity of detection. The optimum flow rate of sample and reagent solutions was surveyed. Diffusivities of biomolecules participated in bioluminescent reaction and consumption rate of ATP were considered as major factors affecting the optimum flow rates. The system can be applied for rapid detection of microorganisms and for monitoring of the health of culture. |
| 저자 |
히에우투언1, 김영범1, 윤주영2, 장우진1, 구윤모1, 김진환2, 김은기1
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| 소속 |
1인하대, 2M&B GreenUs |
| 키워드 |
microfluidic device; bioluminescence; ATP analysis
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| E-Mail |
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| 원문파일 |
초록 보기 |
