| 학회 |
한국화학공학회 |
| 학술대회 |
2003년 가을 (10/24 ~ 10/25, 한양대학교) |
| 권호 |
9권 2호, p.2119 |
| 발표분야 |
생물화공 |
| 제목 |
High-level production of spider silk protein by fed-batch cultivation of recombinant Escherichia coli coexpressing E. coli dead gene and its purification |
| 초록 |
Silk proteins from Nephila clavipes are fibrous proteins containing repetitive sequences with both crystalline and amorphous domains. The synthetic genes had contiguous units of the consensus repeat sequence of the silk protein were constructed in a tandem multimeric form. Silk proteins were expressed in Escherichia coli BL21(DE3) under the strong inducible T7 promoter. For efficient production of silk proteins with large multimers, coexpression of DeaD-box protein was also carried out to increase stability of long mRNA transcripts by synchronized transcription and translation. In order to obtain high-level production of silk protein in large amounts, pH-stat fed-batch cultures were carried out. Finally silk protein was simply purified using IMAC method. |
| 저자 |
이석재, 채송화, 이상엽
|
| 소속 |
한국과학기술원 |
| 키워드 |
silk protein; recombinant protein; high-cell density |
| E-Mail |
|
| 원문파일 |
초록 보기 |
