| 초록 |
We adopted PAMAM G4 as a template polymer and conjugated amino acids on its surface. First, GABA and Arg were conjugated to enhance hydrophobicity/flexibility and transfection efficiency, respectively. Second, oligopeptide of GFLG sequence was used as an enzyme-cleavable linker which should be degraded in lysosome/endosome compartment. Each synthesis step was performed using HOBt/HBTU and DIPEA. The synthesized cationic polymer was evaluated to confirm the formation with plasmid DNA by agarose gel retardation and picogreen reagent assay. Transfection efficiency of the synthesized products were carried out by protein assay and luciferase assay. As results, we can confirm that PAMAM G4 conjugated with GABA and Arg showed lower cytoxicity than PEI 25kDa. Particularly, PAMAM G4-GABA-R displayed higher transfection efficiency than other polymers. |
