| 학회 | 한국공업화학회 |
| 학술대회 | 2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 22권 2호 |
| 발표분야 | 생물공학_포스터 |
| 제목 | Engineered Enterobacter aerogenes to 2,3-butanediol production with lignocellulic biomass as carbon source |
| 초록 | The pathway engineering of Enterobacter aerogenes was attempted to improve its production capability of 2,3–butanediol from lignocellulosic biomass. In the medium containing glucose and xylose mixture as carbon sources, the gene deletion of pflB improved 2,3-butanediol carbon yield by 40%, while the deletion of ptsG increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. The constructed strain, EMY-22-galP, overexpressing glucose transporter (galP) in the triple gene knockout E. aerogenes, ldhA, pflB, and ptsG, provided the highest 2,3-butanediol titer and yield at 12 hr flask cultivation. After pretreatment, the resulting hydrolysate was used as a carbon source for 2,3-butanediol production. After 72 hr in fermentation, the yield of 0.395 g/g sugar was achieved, suggesting an economic production of 2,3-butanediol was possible from lignocellulosic biomass with the metabolically engineered strain. |
| 저자 | 김덕균, 오민규 |
| 소속 | 고려대 |
| 키워드 | Enterobacter aerogenes; Sugarcane bagasse; 2; 3-Butanediol |
