| 초록 |
Acetate is a promising abundant carbon source to achieve cost-effective fermentation. We engineered an Escherichia coli strain to produce itaconic acid from acetate. Since acetate is known to inhibit cell growth, we initially screened for a strain with a high tolerance to 10 g/L of acetate in the medium, and the W strain was selected as the host. Subsequently, the WC strain was obtained by overexpression of cad. However, the WC strain produced only a small amount of itaconic acid because of low acetate uptake. Hence, the acetate assimilating pathway and glyoxylate shunt pathway were amplified for increased acetate consumption and itaconic acid production. The amplification of glyoxylate shunt pathway, especially aceA, seemed remarkably effective with gltA overexpression. The resulting strain, WCIAG4 showed significantly increased acetate assimilation and itaconic acid production, 3.57 g/L. These show that acetate can be a potential feedstock for fermentation with E. coli |
