| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Development of riboswitch-based sensor devices that specifically respond to intracellular concentration of naringenin through in vitro and in vivo selection |
| 초록 |
Measuring Intracellular concentration of metabolites enables facile control of the pathway gene expression and screening or selection for improved producer strains. Transcription factor-based sensors are often accompanied by off-target effect and delayed response which result from trans-regulatory mechanism. On the contrary, riboswitch could bypass the aforementioned problems. Here we demonstrate the process for developing riboswitches which specifically sense naringenin by modulating concentration of naringenin during enrichments and the sequence of negative and positive selections. We found that selected riboswitches work at useful operational ranges which are dependent on the screening condition applied to the library. Finally, we validated the specificity of the riboswitches for naringenin against other flavonoids. We expect that these new riboswitches could be applied to diverse applications. |
| 저자 |
김민선, 장성호, 장성연, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
Riboswitch; Naringenin; Synthetic Biology; Metabolic Engineering
|
| E-Mail |
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