| 학회 |
한국화학공학회 |
| 학술대회 |
2021년 봄 (04/21 ~ 04/23, 부산 BEXCO) |
| 권호 |
27권 1호, p.448 |
| 발표분야 |
생물화공 |
| 제목 |
Development of Robust Glycolic Acid-Producing E. coli strain through Evolutionary and Metabolic Engineering |
| 초록 |
Existing engineered GA-producing strains have limited substrate consumption capacity due to carbon catabolite repression (CCR). Herein, E. coli utilizing D-xylose via Dahms pathway was further engineered to allow cellobiose uptake by expressing cep94A gene from S. degredans. Unlike glucose, cellobiose does not repress D-xylose uptake and metabolism as its intracellular degradation detours CCR resulting in maximum yield of 0.51 g GA g-1 xylose. For a rapid cellobiose consumption, the GA-producing strain was subjected to adaptive laboratory evolution leading to the development of industrially-competitive GA-producing E. coli strain. This work was supported by the National Research Foundation of Korea (NRF) under the Basic Science Research Program through the Ministry of Education (2018R1D1A1B07043993 and 2020R1A6A1A03038817), and by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) funded by the Ministry of Trade, Industry & Energy (MOTIE, No. 20194010201750). |
| 저자 |
Angelo Banares1, Grace Nisola2, 이원근3, 정욱진1
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| 소속 |
1Environmental Waste Recycle Institute (EWRI), 2Department of Energy Science and Technology (DEST), 3명지대 |
| 키워드 |
Biochemical Engineering |
| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
