| 초록 |
RNA interference technology can be used as a treatment for malignant tumor cells, often known as cancer, because it can induce apoptosis by targeting specific cells while minimizing direct effects on body gene maps. However, there is still a problem of delivering siRNA to target cells because of the large reactivity of the RNA compared to DNA that is stable in vivo. The methods that use a polymer or lipid for siRNA has been introduced, but there is no standardized method because of consideration of cytotoxicity in vivo or difficulty of synthesis. In this study, we introduced a method of simply using calcium ion as a transfection agent for short-interference RNA nanoparticles(NP). Calcium has the advantage that it is not necessary to consider the toxicity, in vivo, since it is already existed in the body for bone formation or intracellular osmotic pressure control. We coated siRNA NP with calcium ion and then analyzed the physical properties of particles and carried out in vitro test. |
