| 학회 |
한국화학공학회 |
| 학술대회 |
2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 |
9권 1호, p.543 |
| 발표분야 |
생물화공 |
| 제목 |
Induction charcateristics of Escherichia coli nar promoters in gram-negative host strains |
| 초록 |
The Escherichia coli nar promoter is maximally induced under anaerobic conditions in the presence of nitrate ion or under anaerobic only conditions. Previously we found that the E. coli nar promoter has some desirable characteristics as an inducible promoter in the E. coli host strains. In this study, the E. coli nar promoter with lacZ gene at the downstream was cloned onto a broad-host-range gram-negative vector, pBBR122. It was then induced in some other gram-negative host strains, such as Agrobacterium, Pseudomonas and Rhizobium. From shake-flask and bioreactor experiments, it was found that the E. coli nar promoter was maximally induced under microaerobic conditions in both Agrobacterium and Pseudomonas. Therefore, the E. coli nar promoter can be used as an oxygen-dependent inducible promoter in both Agrobacterium and Pseudomonas host strains, as well as in E. coli host strains. |
| 저자 |
하성진1, 조무환1, 이길호1, 김은정1, 이종원2, 장호남3, 정태완1
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| 소속 |
1영남대, 2대구가톨릭대, 3한국과학기술원 생명화학공학과 |
| 키워드 |
대장균 nar 프로모터; 산소의존형; 그램음성균; 유도특성
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| E-Mail |
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