| 학회 |
한국화학공학회 |
| 학술대회 |
2002년 가을 (10/24 ~ 10/26, 서울대학교) |
| 권호 |
8권 2호, p.4089 |
| 발표분야 |
생물화공 |
| 제목 |
표면플라즈몬 공명현상을 이용한 Escherichia Coli O157:H7 검출용 면역센서 |
| 초록 |
Immunosensor based on surface plasmon resonance (SPR) using self-assembled protein G layer was developed for the detection of Escherichia coli O157:H7. A self-assembled protein G layer on gold (Au) surface was fabricated by adsorbing the mixture of 11-mercaptoundecanoic acid (MUA) and hexanethiol with various molar ratio and the activation process for chemical binding between free amine (-NH2) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of self-assembled protein G layer on Au substrate and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The surface morphology analyses of self-assembled protein G layer on Au substrate, monoclonal antibody (Mab) against E. coli O157:H7 immobilized on protein G and bound E. coli O157:H7 extracts on immobilized Mab against E. coli O157:H7 were performed by atomic force microscope (AFM). The detection limit of immunosensor based on SPR for the detection of E. coli O157:H7 was could find up to 104 cells/mL. |
| 저자 |
오병근, 김영기, 배영민, 이우창, 이원홍, 최정우
|
| 소속 |
서강대 |
| 키워드 |
Escherichia coli O157:H7; Immunosensor; Protein G; Self-assembled layer; Surface plasmon resonance
|
| E-Mail |
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| 원문파일 |
초록 보기 |
