| 초록 |
DPN, a scanning-probe-based lithography in which an AFM tip is used to generate nanoscale chemical patterns by directly transferring molecules to a surface, offers a number of realized and potential advantages over other nanofabrication methods. The method has been used to create nanometer-scale patterning based on small organic molecules as well as biological macromolecules such as proteins, oligonucleotides, peptides, and viruses. In this work, we have patterned E.coli bacterial cells on LB-agar substrate via DPN technique by using poly(2-methyl-2-oxazoline)(PMeOx)-coated AFM probe which was fabricated by ring opening polymerization of 2-methyl-2-oxazoline monomers on the probe surface. We demonstrate that one can control the number of bacterial cells deposited on the surface by varying the viscosity of the ink solution with glycerol. Furthermore, we show that the patterned bacterial cells can be proliferated on LB-agar substrate on the appropriate incubation conditions. |
