| 초록 |
Microchip electrophoresis are useful methods for genetic analysis by the benefit of rapid analysis, small amount of sample/reagent consumption, and portability. Capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) analysis is one of promising DNA detection method due to its simplicity and versatility. In non-denaturing conditions, single-strand DNA forms a folded conformation that is determined by its sequence. In SSCP analysis, electrophoretic mobility of DNA fragment is influenced by its folded shape and size. Theoretically, any DNA sequence variation can be detected with SSCP electrophoretic mobility pattern. However, low separation resolution is a critical limitation of SSCP analysis on microchip. In this study, we improved separation resolution via microchannel width control. As a result, this microchip-SSCP analysis system was successfully applied to the pathogen detection and SNP marker detection. |
