| 초록 |
High level secretory production of human granulocyte-colony stimulating factor (hG-CSF) by fed-batch cultures of Escherichia coli MC4100 harboring plasmid pTHKCSFmII was investigated. Induction at higher cell density (late-exponential phase) was more efficient than induction at lower cell density (early-exponential phase and mid-exponential phase) for the hG-CSF production. Among three different feeding strategies (pH-stat, exponential, and constant feeding), the constant (0.228 g glucose/min) and exponential feeding strategies (specific growth rate of 0.116h-1) were better than the pH-stat for hG-CSF production. Therefore, an optimal condition for hG-CSF production was to induce at higher cell density phase using constant (0.228g glucose/min) or exponential (specific growth rate of 0.116h-1) as a feeding strategies. Under these conditions, 4.35g/L of hG-CSF protein could be produced in a secretory form. |
