| 학회 | 한국화학공학회 |
| 학술대회 | 2013년 봄 (04/24 ~ 04/26, 광주 김대중컨벤션센터) |
| 권호 | 19권 1호, p.423 |
| 발표분야 | 생물화공 |
| 제목 | Substrate-mediated viral gene delivery system by catecholamine immobilized surfaces |
| 초록 | Gene therapy is a therapeutic method to treat human disease by modifying the genes of cells. One of the important criteria of a successful gene therapy is the efficiency of gene delivery with safety. Recently, lots of protocols have been developed for gene delivery systems using viral and non-viral vectors. AAV is widely used for the viral gene delivery with safety and efficiency. However, it may have limitations in the stimulation of stem cells due to its low transfection efficiencies. In this study, we developed substrate-mediated viral gene delivery system with adhesive materials using for macro-patterned gene delivery by “pipet drawing”. We used sticky viruses for patterned gene delivery system to various cell lines especially human neural stem cells. Development of gene delivery system to stem cells has an importance in the gene therapy and tissue engineering applications, due to its enormous potential to threat many diseases which have no effective therapy until now. |
| 저자 | 김은미, 박민아, 이슬기림, 김정숙, 진규형, 박국인, 장재형 |
| 소속 | 연세대 |
| 키워드 | AAV; Gene therapy; Catecholamine; Human neural stem cell |
| 원문파일 | 초록 보기 |
