| 초록 |
Due to the increasing significance of genetic marker in diagnosis, efficient genetic marker detection system is necessary. Capillary electrophoresis-based single-strand DNA conformation polymorphism analysis (CE-SSCP), which separates DNA molecules by conformational difference of ssDNA and quantifies them by fluorescence intensity, can detect multiple genetic markers quantitatively. We have demonstrated CE-SSCP could be used to detect multiple pathogen targets quantitatively by its genomic regions of 16S rRNA gene or 16S rRNA itself. Moreover, multiplex mRNA quantification was also demonstrated for seven mRNAs involved in the central carbon metabolic pathway, and this result showed that expression of marker genes can be monitored by this method. Additionally, conventional polymer matrix, which is the major component of CE separation, was replaced by PEO-PPO-PEO tri-block copolymer to improve resolution. Examples will be shown for the potentials in the various applications such as copy number variations, single nucleotide polymorphism, and expression analysis. |
