| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1570 |
| 발표분야 |
생물화공 |
| 제목 |
A Nobel Detection Method of Specific Antigen Based on Nanomaterial Combined with Viral Nanoparticles |
| 초록 |
The biosensor systems that are subjected to detecting protein analytes requires immobilization of probe antibodies on the surface of nanomaterial, which is a key step determining sensitivity. In this study, we used the HBV capsid-derived and biotinylated nanoparticles that are subjected to attaching to avidin molecules on the surface of nanostructured quartz. Owing to the surface-displayed tandem repeat of B domain of Staphylococcal protein A of the viral particles, the nanostructured quartz surface is densely covered by probe antibodies with controlled orientation, leading to the preparation of 3-dimensional assay system. We developed a highly sensitive biosensor to detect the E. coli enterotoxin marker. By combining the engineered viral particles derived from HBV capsid with the nanostructured quartz, we constructed a novel 3-dimensional assay system and successfully detected the target enterotoxin marker even at attomolar concentrations, suggesting that sensitive diagnosis may be possible at very early stage of ETEC infection. |
| 저자 |
이종환, 이지원, 권구철, 박진승, 서혁성, 안금영, 이대성, 송종암, 이은정
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| 소속 |
고려대 |
| 키워드 |
biosensors; quartz;
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| E-Mail |
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| 원문파일 |
초록 보기 |
