| 초록 |
A method for capture, analysis, and sorting of live cells using a photodegradable hydrogel (photogel) functionalized with antibodies will be demonstrated. Photogel substrates used to first isolate human CD4 or CD8 T-cells from a heterogeneous cell suspension and then to release desired cells or groups of cells by UV-induced photodegradation. Flow cytometry analysis of the retrieved cells revealed approximately 95% purity of CD4 and CD8 T-cells, suggesting that this substrate had excellent specificity. To demonstrate the possibility of sorting cells according to their function, photogel substrates that were functionalized with anti-CD4 and anti-TNF-a antibodies were prepared. Single cells captured and stimulated on such substrates were identified after immunofluorescent staining and could be retrieved by site-specific exposure to UV light through a microscope objective. The use of a photodegradable gel allows the detection of cells of interest based on their function and the release of these cells for downstream analysis, which could entail gene expression studies or continued cultivation. |
