| 초록 |
A psychrophillic bacterium designated as Pseudoalteromonas sp. L203 based on its physiological properties and 16S rRNA sequence was isolated from the bottom sediment of Marian cove, King George island, Antarctica. This strain optimal growth temperature of about 15oC was found to produce extracellular phospholipase A1. The production of this psychrophillic enzyme was optimized by addition of egg-york in th emedium and the purification yield wa simproved using hydrophobic-affinity column chromatography. Using this psychrophillic phospholipase, phosphatidyl cholines with EPA at sn-1 and sn-2 position were produced from ethyl ester of EPA and lyso-phosphatidyl choline in two phase reaction system was also optimized to a yield of 35%. With this enzyme and other phospholipases from marine bacteria such as Streptomyces sp.3424 (PLC) and Streptomyces sp.2436 (PLA2), an enzymatic phospholipid manupulation set can be constructed. |
