| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 |
18권 1호, p.437 |
| 발표분야 |
생물화공 |
| 제목 |
Improved Carbon Dioxide Fixation and Organic Compounds Production by Inducible Expression of the Carbonic Anhydrase and Phosphoenolpyruvate Carboxylase in Rhodobacter sphaeroides |
| 초록 |
Carbonic anhydarse (CA) is as zinc-containing metalloenzyme catalyzing the reversible hydration of CO2 to HCO3-. Phosphoenolpyruvate (PEP) carboxylase (PEPC) is an enzyme involved in carbon metabolism that catalyzes the fixation of CO2 to PEP in the presence of HCO3- to yield oxaloacetate and inorganic phosphate. Therefore, CA and PEPC is a key factor for the biological fixation of CO2 and enhanced production of organic compounds. In this study, Rhodobacter sphaeroides was used as a recipient strain for transformation with the plasmid pBBR1mcse-2 to induce over-expression of CA and PEPC respectively. This result showed that cells used more CO2 and enhanced the production of organic compounds under anaerobic light conditions. The function of CA and PEPC in R. sphaeroides would accelerate the biocarbonate-CO2 conversion in the intracellular under photosynthetic conditions. Therefore, this study will prove useful in efforts to improve CO2 fixation and photosynthetic ability in this species for a variety of biotechnological applications. |
| 저자 |
박주용1, 김양훈2, 민지호1
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| 소속 |
1전북대, 2충북대 |
| 키워드 |
carbon dioxide fixation; carbonic anhydrase; phosphoenolpyruvate carboxylase; Rhodobacter sphaeroides
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| E-Mail |
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| 원문파일 |
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