| 초록 |
Polymersomes composed of hydrophilic cores and hydrophobic shells have significant storage capacity, providing the synergistic effects among various therapeutic agents (drug or siRNA/shRNA) with different pharmacokinetics. A series of methoxy-polyehtylene glycol (mPEG)-block copolymer of poly(D,L-lactic acid) (PLA) with varying mass fractions (0.2-0.4) of mPEG were synthesized by ring opening polymerization. The morphology of the polymersomes formed by the thin film hydration method was analyzed by cryo-TEM and their loadable capacities were confirmed by SEM and CLSM, respectively. In vitro cell viability of synchronous gene- and drug-loaded polymersomes showed that they more inhibit proliferation of human gastric cancer cells (MKN-45 and MKN-28) than only gene- and drug- loaded ones. These results demonstrate that co-delivery of gene and drug using polymersomes could achieve a synergistic effect compared to individual treatments of single gene and/or drug. |
