| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
대학원생 구두발표 |
| 제목 |
Lipid-like nanoparticles for small interfering RNA delivery for treatment of viral hepatic diseases |
| 초록 |
Delivery of small interfering RNA (siRNA) is an emerging approach of treating diverse destructive diseases. Here, we report the use of lipid-like synthetic molecules, named lipidoids, as siRNA carriers for treatment of hepatitis C virus (HCV). Several lipidoids were synthesized by conjugation addition of alkyl-acrylamides to amines. Through a reporter siRNA delivery, we have identified an effective lipidoid for siRNA delivery to human hepatic cells. The lipidoid was then used to transfect siRNA targeting PRK2, an endogenous enzyme which is required for HCV replication. Significant knockdown of PRK2 expression was observed in the transfected human hepatic cells. HCV replication in HCV-infected hepatic cells was significantly suppressed by lipidoid-mediated PRK2 siRNA delivery. This lipidoid will be tested for its delivery efficacy to liver tissue in future study. This work was supported by a grant (10035159-2010-01) funded by the Ministry of Knowledge Economy, Korea. |
| 저자 |
박현지1, 김태은1, 김은정1, 강성민2, 이해신2, 오종원1, 조승우1
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| 소속 |
1연세대, 2한국과학기술원 화학과 |
| 키워드 |
siRNA; nanoparticle; delivery; HCV
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| E-Mail |
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