| 초록 |
The achievement of tissue engineering can be highly depending on the capability to generate complicated, cell seeded three dimensional (3D) micro/nano-structures. So, various fabrication techniques that can be used to precisely design the architecture and topography of scaffolding materials will signify a key aspect of multi-functional tissue engineering. Previous methods for obtaining scaffolds based on top-down are often not satisfactory to produce complex micro/nano-structures due to the lack of control on scaffold architecture, porosity, and cellular interactions. However, the 3D printing method can be used to design sophisticated 3D tissue scaffolds that can be engineered to mimic the micro/nano-architecture of tissues, using computer aided approach. Also, in recent, the method has been modified and optimized to fabricate 3D scaffolds using various natural biopolymers (collagen, alginate, and chitosan etc.). Variation of the structure and polymer concentration allowed tailoring the physical and biological properties of the scaffolds. In this presentation, the 3D printing methods including a cell-printing process for attaining highly bioactive and functional scaffolds for tissue engineering applications will be introduced. Moreover, various in vivo and in vitro results will show that the fabricated scaffolds can carry out their structural and biological functionality. |
