| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 봄 (04/22 ~ 04/23, 대구 EXCO) |
| 권호 |
16권 1호, p.831 |
| 발표분야 |
재료 |
| 제목 |
Release of BSA-FITC incorporated in calcium phosphate microsphere |
| 초록 |
This work presents release of BSA-FITC incorporated in CaP microsphere depending on storage time by measuring absorbance of supernatants at 280nm or performing micro BCA protein assay.Calcium phosphate microspheres were prepared by emulsification (water-in-oil) and subsequent nucleation. The emulsification and nucleation were simulated by pH change due to enzyme reaction which makes pH vary from 4 to 7. In order to find optimum condition to form the microsphere, we varied ratio of enzyme to substrate. Obtained BSA-FITC incorporated calcium phosphate microspheres are shown the homogeneous particles sizes in the range of 200-300㎛ in SEM images. We conformed decomposition of urea by urease using FT-IR and incorporation of BSA-FITC into the microsphere using confocal fluorescent microscope. The BSA-FITC incorporated CaP microspheres were stored at 37 ℃ for one month. During the storage, we took 2 mL of supernatant each sampling time for analyzing release of BSA-FITC from the microsphere. We believe that these materials could be potentially applied to the biomedical fields as an injectable bone filler with the controlled drug release materials. |
| 저자 |
고일환1, 이진형2, 장정호3
|
| 소속 |
1Bio-IT Convergence Center, 2Korea Institute of Ceramic Engineering and Technology / Department of Chemical Engineering, 3Inha Univ. |
| 키워드 |
Drug delivery; bone pillar; CaP
|
| E-Mail |
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| 원문파일 |
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