| 초록 |
M. extorquens uses C1 compounds as a sole carbon source, so it has been in spotlight. Formate could be synthesized from CO2, regarded as environmental, sustainable C1 feedstock. In this study, we produced lycopene from formate using M. extorquens by engineering non-mevalonoate pathway, also called MEP pathway, for effective lycopene production. First, LMZ01 strain was constructed by deleting CrtCD, genes for consuming lycopene, and used as a parental strain, showing improved lycopene production. Then, dxs, a rate-limiting step of MEP pathway, was overexpressed under lac, tac, and mxaF promoter, respectively. Expression level was shown to be strong in the order of mxaF, tac, and lac promoter by expressing EGFP. Interestingly, while the mxaF promoter with strong expression level was ineffective, the tac promoter with moderate level showed 45% increase in lycopene production. This showed lycopene could be produced reasonably from formate and moderate expression of MEP pathway is crucial. |
