화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.30, No.8, 1620-1625, August, 2013
DOI10.1007/s11814-013-0102-z  Export Citation
Loofah sponge activated by periodate oxidation as a carrier for covalent immobilization of lipase
Renmin Gong1, 2, †, Jian Zhang2, Jun Zhu2, Jianting Wang2, Qiumei Lai2, and Bo Jiang1
  • 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China
  • 2College of Life Science, Anhui Normal University, Wuhu 241000, P. R. China
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Cellulose in loofah sponge was first oxidized by sodium periodate at positions 2 and 3 of anhydroglucose unit to introduce aldehyde groups, which were able to react with amino groups of lipase to form Schiff’s base, and result in loofah sponge immobilized lipase. The result of periodate oxidation was confirmed by determination of aldehyde group content and analysis of Fourier transform infrared spectra. The optimal pH for both free and immobilized lipases was approximately 7.5. The maximum activity of immobilized lipase was observed at 40 ℃, while the free lipase exhibited maximum activity at 37 ℃. The immobilized lipase presented improved thermal stability, storage stability, and reusability.
Keywords:Loofah Sponge;Periodate Oxidation;Lipase;Immobilization
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