The present study, deals with a new mixing technique using a two-phase electrode array, which is charged with alternating current (AC) signals, located in specific parts of the geometry. This significantly contributes to a chaotic mixing mechanism using a low amplitude AC voltage within a micro-channel. Study analysis demonstrates that the optimization of the effective parameters such as geometrical features, voltage amplitude, fluid inlet velocity, AC frequency and phase lag for a defined fluid can lead to an optimum and highly efficient mixer by considerably increasing disturbances in a primary highly ordered laminar flow. Three different geometries of micro mixer are studied; one-ring type, diamond type and two-ring type and it is shown that the optimum mixing quality of 99.4, 98 and 99.8% are achievable for them, respectively. Finally all the aforementioned studies have also been done for the case of blood as a biological fluid. (C) 2016 Elsevier B.V. All rights reserved.