In vitro 3D skin model using gelatin methacrylate hydrogel

Bong Shin Kwak; Wonho Choi; Joong-won Jeon; Jong-In Won; Gun Yong Sung; Bumsang Kim; Jong Hwan Sung

Journal of Industrial and Engineering Chemistry, Vol.66, 254-261, October, 2018

DOI10.1016/j.jiec.2018.05.037 Export Citation

In vitro 3D skin model using gelatin methacrylate hydrogel

Bong Shin Kwak, Wonho Choi, Joong-won Jeon, Jong-In Won, Gun Yong Sung¹, Bumsang Kim^†, and Jong Hwan Sung^†

Department of Chemical Engineering, Hongik University, Seoul, 04066, Republic of Korea
¹Cooperative Course of Nano-Medical Device Engineering, Department of Materials Science and Engineering, and Integrative Materials Research Institute, Hallym University, Chuncheon, 24252, Republic of Korea

E-mail:,

Interests in in vitro skin models have been growing. Collagen, which is a main scaffold material for in vitro 3D skin models, has weak mechanical properties, often resulting in undesirable contraction. The physiological characteristics of the skin models often depend on the matrix in which cells are cultured. In this study, we developed a 3D skin model using gelatin methacrylate. The mechanical and transport properties were studied, and attachment and growth of fibroblasts and keratinocytes were examined. Fibroblasts preferred softer matrix, whereas HaCaT cells preferred harder matrix of gelatin methacrylate. This study provides information for developing in vitro skin models.

Keywords:In vitro skin models;Gelatin methacrylate;Extracellular matrix;Fibroblasts;Keratinocytes

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[4] Matsusaki M, et al., J. Biomed. Mater. Res. A, 103(10), 3386 (2015)

[5] Rhee S, Exp. Mol. Med., 41(12), 858 (2009)

[6] Chau DYS, et al., Biofabrication, 5(3) (2013)

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[9] Sun T, et al., J. Biotechnol., 122(3), 372 (2006)

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[13] Lee SH, Shim KY, Kim B, Sung JH, Biotechnol. Prog., 33(3), 580 (2017)

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[23] Nichol JW, et al., Biomaterials, 31(21), 5536 (2010)

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[25] Lee AG, Arena CP, Beebe DJ, Palecek SP, Biomacromolecules, 11(12), 3316 (2010)

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