The separation of glucose and fructose, which is very difficult because of their physico-chemical similarity, was attempted using a temperature cycling zone adsorption that does not need eluent in ion exchange chromatography. The separation of fructose was studied by heating ion exchange column to 5℃ and 50℃ alternately, using the fact that the adsorptive capacity of glucose to an ion exchange resin does not depend much on temperature while that of fructose is more sensitive to temperature. The separability was represented by the function of the period of temperature change and the flow rate of feed solution and it was maximal when the period of temperature change was twice as nuch as the residence time of the feed solution in the column. The separation factor decreased from 1.15 to 1.0 with the increase of feeding solution concentration from 1.5% to 5%. In order to reduce the temperature gradient within the column a bundle of small multicolumns were adopted to show the increase in the separability.